Bioengineering at the micro-scale / Susanna Maria Lladó Maldonado (2019)

Public ISBD Titre : Bioengineering at the micro-scale : design, characterization and validation of microbioreactors Type de document : document électronique Auteurs : Susanna Maria Lladó Maldonado, Auteur Editeur : Gottingen [Allemagne] : Cuvillier-Verlag Année de publication : 2019 Collection : Schriftenreihe des institutes fur bioverfahrenstechnik der technischen universität braunschweig num. Band 81 Importance : 1 fichier PDF (3.8 Mo) ISBN/ISSN/EAN : 978-3-7369-6025-1 Note générale : Mode d'accès : accès au texte intégral par : - authentification après inscription à la plateforme EBSCOhost ou - adresse IP de l'École. Bibliogr. p. 97-106 Langues : Anglais (eng) Mots-clés : Biotechnology Bioengineering Bioreactors Index. décimale : 606 Application de la biotechnologie Résumé : The miniaturisation of bioreactors to the microliter-scale and the integration of online sensors for monitoring the most important process variables during the cultivations is a promising approach for the screening and optimization of cultivation and biocatalysis processes, enabling information-rich, parallelised and cost-effective experiments under well-controlled environmental conditions. The main advantages of microbioreactors (MBRs) are minimization of space and reagents, their easy manipulation, and their high-throughput screening potential, which make them very interesting tools to develop bioprocesses. This thesis is focused on the development of MBRs and the sensor integration for monitoring optical density (OD), dissolved oxygen (DO), pH and glucose as well as their validation for different biotechnological applications. On the basis of a developed PDMS-based MBR, a borosilicate glass-based microbubble column-biroeactor (gμBC) (working volume of 60 μL, aeration occurs through a nozzle with Ø =26 μm) was designed and manufactured by wet etching and powder blasting technology. The gµBC proved to have good oxygen transfer capacity, reaching kLa values up to 320 1/h and fast mixing times θ95 down to 5.5 s when working at a gas superficial velocity uG of 2.25∙10-3 m/s. The mixing performance was simulated using a simplified CFD model, and the tracer profile yielded a good qualitative prediction that was comparable to the experimental results, presenting a tolerable deviation of the mixing times. The gµBC was validated as a suitable cultivation screening tool with a batch cultivation of Saccharomyces cerevisiae with the real-time online monitoring of OD and DO. Furthermore, a cuvette-based microbubble column-bioreactor (cμBC) made of polystyrene (working volume of 550 μL, aeration occurs through a nozzle with Ø ≤ 100 μm) was developed and manufactured with online sensors for pH, OD, DO and glucose. The cμBC showed homogeneous mixing of the cultivation medium with θ95 Note de contenu : Summary : 1. Introduction, objectives and thesis outline 2. Theoretical background 3. Materials and methods 4. Characterization of a multiphase microbioreactor 5. Online sensor integration of a multiphase microbioreactor 6. Application of microbioreactors 7. Conclusions and outlook Bioengineering at the micro-scale : design, characterization and validation of microbioreactors [document électronique] / Susanna Maria Lladó Maldonado, Auteur . - Gottingen [Allemagne] : Cuvillier-Verlag, 2019 . - 1 fichier PDF (3.8 Mo). - (Schriftenreihe des institutes fur bioverfahrenstechnik der technischen universität braunschweig; Band 81) . ISBN : 978-3-7369-6025-1 Mode d'accès : accès au texte intégral par : - authentification après inscription à la plateforme EBSCOhost ou - adresse IP de l'École. Bibliogr. p. 97-106 Langues : Anglais (eng) Mots-clés : Biotechnology Bioengineering Bioreactors Index. décimale : 606 Application de la biotechnologie Résumé : The miniaturisation of bioreactors to the microliter-scale and the integration of online sensors for monitoring the most important process variables during the cultivations is a promising approach for the screening and optimization of cultivation and biocatalysis processes, enabling information-rich, parallelised and cost-effective experiments under well-controlled environmental conditions. The main advantages of microbioreactors (MBRs) are minimization of space and reagents, their easy manipulation, and their high-throughput screening potential, which make them very interesting tools to develop bioprocesses. This thesis is focused on the development of MBRs and the sensor integration for monitoring optical density (OD), dissolved oxygen (DO), pH and glucose as well as their validation for different biotechnological applications. On the basis of a developed PDMS-based MBR, a borosilicate glass-based microbubble column-biroeactor (gμBC) (working volume of 60 μL, aeration occurs through a nozzle with Ø =26 μm) was designed and manufactured by wet etching and powder blasting technology. The gµBC proved to have good oxygen transfer capacity, reaching kLa values up to 320 1/h and fast mixing times θ95 down to 5.5 s when working at a gas superficial velocity uG of 2.25∙10-3 m/s. The mixing performance was simulated using a simplified CFD model, and the tracer profile yielded a good qualitative prediction that was comparable to the experimental results, presenting a tolerable deviation of the mixing times. The gµBC was validated as a suitable cultivation screening tool with a batch cultivation of Saccharomyces cerevisiae with the real-time online monitoring of OD and DO. Furthermore, a cuvette-based microbubble column-bioreactor (cμBC) made of polystyrene (working volume of 550 μL, aeration occurs through a nozzle with Ø ≤ 100 μm) was developed and manufactured with online sensors for pH, OD, DO and glucose. The cμBC showed homogeneous mixing of the cultivation medium with θ95 Note de contenu : Summary : 1. Introduction, objectives and thesis outline 2. Theoretical background 3. Materials and methods 4. Characterization of a multiphase microbioreactor 5. Online sensor integration of a multiphase microbioreactor 6. Application of microbioreactors 7. Conclusions and outlook

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Concepts in biotechnology / Buchholz, Klaus (2010)

Public ISBD Titre : Concepts in biotechnology : history, science and business Type de document : texte imprimé Auteurs : Buchholz, Klaus, Auteur ; Collins, John, Auteur Editeur : New York : John Wiley & Sons Année de publication : 2010 Importance : 471 p. Présentation : ill. Format : 24 cm ISBN/ISSN/EAN : 978-3-527-31766-0 Note générale : Bibliogr. en fin de chapitres. - Index Langues : Anglais (eng) Mots-clés : Bio-industries Biotechnologie Index. décimale : 606 Application de la biotechnologie Résumé : The authors present a systematic, yet concise, overview of the field, science and application, of biotechnology and how it developed. This includes some history, with roots going back several thousand years, highlighting great achievements in the 19th and early 20th centuries. It illustrates how progress actually occurs, driven by ideas and human activity with individuals playing decisive roles. The traditional fields of biochemical engineering and industrial biotechnology, as well the most recent progress in biosystems engineering, modern pharmaceutical and plant biotechnology are covered. The recent dramatic explosion in knowledge from genetics and cell biology have revolutionized biotechnology causing fundemental changes in related medical fields: e.g., from intervention to preventive and regenerative medicine. How this occurred, the prime movers, the principle developments, the concepts, science and applications are described. As the application of biotechnology impacts on society relevant questions in the area of economics, ethics and patenting are adressed in dedicated chapters. The involvement of the authors each with some 40 years as scientists and contemporary witnesses of these events gives the text an historical authenticity which the reader may find make the more recent events come to life. The book should be an introductory guide for students as well as scientists and engineers engaged in, or intending to work in biotechnology. Note de contenu : In symmary : 1. The early period to 1850 2. The period from 1850 to 1890 3. The period from 1890 to 1950 4. Outlook, from 1950 onwards : biotechnology - science or what ? 5. The new paradigm based on molecular biology and genetics 6. The beginnings of the new biotechnology 7. Ethical aspects related to genome research, and reproductive medicine 8. Elucidating protein structure : the beginnings of rational protein design 9. The development of antibodies as pharmaceutical products 10. Hereditary disease and human genome analysis 11. Transgenic animals and plants ... Concepts in biotechnology : history, science and business [texte imprimé] / Buchholz, Klaus, Auteur ; Collins, John, Auteur . - New York : John Wiley & Sons, 2010 . - 471 p. : ill. ; 24 cm. ISBN : 978-3-527-31766-0 Bibliogr. en fin de chapitres. - Index Langues : Anglais (eng) Mots-clés : Bio-industries Biotechnologie Index. décimale : 606 Application de la biotechnologie Résumé : The authors present a systematic, yet concise, overview of the field, science and application, of biotechnology and how it developed. This includes some history, with roots going back several thousand years, highlighting great achievements in the 19th and early 20th centuries. It illustrates how progress actually occurs, driven by ideas and human activity with individuals playing decisive roles. The traditional fields of biochemical engineering and industrial biotechnology, as well the most recent progress in biosystems engineering, modern pharmaceutical and plant biotechnology are covered. The recent dramatic explosion in knowledge from genetics and cell biology have revolutionized biotechnology causing fundemental changes in related medical fields: e.g., from intervention to preventive and regenerative medicine. How this occurred, the prime movers, the principle developments, the concepts, science and applications are described. As the application of biotechnology impacts on society relevant questions in the area of economics, ethics and patenting are adressed in dedicated chapters. The involvement of the authors each with some 40 years as scientists and contemporary witnesses of these events gives the text an historical authenticity which the reader may find make the more recent events come to life. The book should be an introductory guide for students as well as scientists and engineers engaged in, or intending to work in biotechnology. Note de contenu : In symmary : 1. The early period to 1850 2. The period from 1850 to 1890 3. The period from 1890 to 1950 4. Outlook, from 1950 onwards : biotechnology - science or what ? 5. The new paradigm based on molecular biology and genetics 6. The beginnings of the new biotechnology 7. Ethical aspects related to genome research, and reproductive medicine 8. Elucidating protein structure : the beginnings of rational protein design 9. The development of antibodies as pharmaceutical products 10. Hereditary disease and human genome analysis 11. Transgenic animals and plants ...

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